Stable hemoglobin-based biosensor based on coordination-assisted microfluidic technology for hydrogen peroxide determination

Enhancing the stability and sensitivity of electrochemical biosensors is highly significant for their practical application. Herein, inspired by formation mussel foot protein, we proposed a strategy to construct hemoglobin-based biosensor hydrogen peroxide detection using hydrophobic ionic liquid (HIL) coordination assisted microfluidic technology. The active layer HIL@Hb was achieved mixing BBimPF6 Hb via channel, in which HIL helps maintain conformational dynamic mobility hemoglobin (Hb), while process reactor prevents aggregation Hb. Further, electrode surface modified with ultra-thin MXene-Ti3C2 nanosheets ensure effective adhesion collection sensing signals, thus improving sensor synergistic catalysis. Experimental results demonstrate that our designed has good repeatability stability, can retain 93% initial current response after 30 uses about 90.11% its primary H2O2 days. And it linear range 1.996–27.232 μM, limit reaching 1.996 nM (signal-to-noise ratio, S/N = 3), 52.08 μA·μM−1·cm−2. Overall, this research offers facile constructing stable effectively detect peroxide.